R Sreela, KS Rajesh and Savitha Balan

Thaila preparations constitute a major role in treating ailments in Ayurveda. Majority of thaila preparations are used exclusively for external application; but there are some oils which can be used both externally and internally. The external includes thalam, pichu, abhyanga of head and body, vasthi etc. Thaila when used internally after doing avarthana gives more quick relief. Usually preparation of thaila without avarthana is used in large quantity i.e. 5ml/10ml etc. But by avarthana it can be reduced to 5 drops/10 drops. The process of avarthana is done to potentiate the thaila to attain maximum efficacy with minimum dosage. Ksheerabala thaila is one such thaila yoga which can be used both internally and externally and also used in avarthana form. Now most of the Ayurveda pharmacies are preparing the Ksheerabala avarthana and it is very costly also. Present study aims to evaluate the effect of avarthana of Ksheerabala using TLC, HPTLC and also physio-chemical evaluation. Here 1^st to 7^th avarthana were subjected for evaluation. The HPTLC was conducted to detect the presence of alkaloids like pseudo ephedrine & ephedrine and also ecdysteroids like 20-hydroxyecdysone, which are the major chemical constituents of Sida from which Ksheerabala avarthana is prepared. 20-hydroxyecdysone was present in Sida kashaya only. The Ksheerabala avarthana showed an increase in a peak area from 1^st to 7^th avarthana. The peak was identified as the peak of ephedrine by spotting ephedrine standard and it has an Rf of 0.75 in the solvent system Ethyl acetate: cyclohexane: methanol: ammonia-25:15:10:5 (v/v/v). It was found that the 7^th avarthana shows an additional peak as a continuation of the peak of ephedrine at an Rf of 0.82 in the same solvent system. GCMS also reinstates the presence of additional compound in 7^th avarthana. Each of the avarthana showed increasing color intensity in steroidal test. This study also helps to find the authenticity of a sample of Ksheerabala available in the market.

DOI: 10.22271/phyto.2024.v13.i6e.15203

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