A Satheesh Kumar, E Manikgantan and M Ravichandran
Introduction: Traditional Siddha medicine relies on polyherbal formulations like Maramanjal Chooranam (MMC), valued for their medicinal properties. Quality standardization and safety evaluations are essential for these formulations to ensure their therapeutic efficacy and patient safety.
Aim and Objective: This study aims to standardize and assess the quality of MMC through phytochemical analysis and microbiological evaluation, ensuring compliance with AYUSH and WHO standards for herbal formulations.
Materials and Methods: The phytochemical screening was conducted using qualitative tests to identify bioactive compounds of the MMC. To confirm the sterility of the formulation, a pour plate method was employed, followed by pathogen-specific tests on selective media to detect the presence of E. coli, Salmonella, Staphylococcus aureus, and Pseudomonas aeruginosa. Additionally, SEM-EDX analysis was used to examine the elemental composition of MMC.
Results and Discussion: Phytochemical analysis revealed the presence of multiple bioactive compounds like alkaloids, flavanoids, steroids, triterpenoids, coumarin, phenol, tanin, sapopnins, sugar which affirms the therapeutic potential of MMC. The pour plate method showed no microbial growth, indicating a sterile sample and specific pathogen tests confirmed the absence of pathogenic organisms. SEM-EDX analysis identified key elements, including carbon, oxygen, sodium, potassium, and sulfur, along with trace levels of mercury, lead, and arsenic within permissible limits, ensuring adherence to quality standards.
Conclusion: This study confirms that MMC meets standard quality and safety requirements, with no detectable pathogenic contamination and a favorable elemental profile. These findings support the formulation’s safety and potential for therapeutic applications.
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