Priyanka Kumari

Solanum nigrum L. is a medicinal plant of Solanaceae family with ethno importance. It belongs to Eurasia. It is a beneficiary resource of solanine for its therapeutic use. It has been traditionally used for many ailments such as anti-diuretic, anti-inflammation, anti-cancerous etc. and has some anti-oxidant properties also. Callus formation was achieved in the media supplemented with NAA, 2,4-D, IAA, NAA + BAP, 2,4-D + BAP, IAA+BAP used separately and in combination at different concentration (0.5-2.5mg/l). The initiation of callus was observed from different explants of Solanum nigrum L. i.e stem, leaf, nodal stem and flower in 3 weeks of culture. The colour of callus from different explants was observed from white to green in colour. Different colours of callus was seen from explants supplemented with different combination and concentration of phytohormones. During the course of callus development green embryogenic callus was also observed on the surface of initially synthesized callus indicating the presence of pre embyogenic factor (somatic embryogenesis). Further it was also investigated that green callus had the potentiality to develop into shoots.

DOI: 10.22271/phyto.2025.v14.i1c.15242

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