Shani Dominic and Rakesh Kumar Jat

Multidrug resistance in human pathogenic bacteria is a result of commercial antimicrobial agents and synthetic drugs, while plant-derived medicines offer potential therapeutic benefits for treating infectious disorders. The objective of this research was to assess the antibacterial efficacy of the ethanolic extract of Leea indica root. The root extract underwent qualitative analysis to identify chemical ingredients through distinct color alterations, using established protocols and a 10% solution in each test. The roots were quantitatively assessed for its total phenolic content using the Folin-Ciocalteau technique, with gallic acid as the benchmark. Kumaran and Karunakaran's method was used to assess the flavonoid content of a root extract, utilizing spectrophotometer measurements and quantification of quercetin equivalents. The bacterial cultivation and antifungal activity of plant extract against Candida albicans and Streptomycin. Nutrient agar was prepared, and bacterial suspensions were spread over the surface. Filter paper discs were saturated with the extract and standard drug, and incubated at 37 °C for 24 hours. The extract showed significant antibacterial and antifungal activities compared to standard drugs Gentamycin and Clotrimazole. The total phenolic content was 37.66±0.125 mcg/mL, and the total flavonoid content was 20mcg/mL. However, the alcoholic extract showed no substantial antifungal efficacy against Candida albicans. The study found that Leea indica roots may be more effective as an antimicrobial agent than Gentamycin, despite not showing significant antifungal activity against Candida albicans.

DOI: 10.22271/phyto.2025.v14.i1e.15259

Pages: 368-372  |  228 Views  98 Downloads