Rispa Johns, Anuranjini NA, Joseph Thomas K, Niranjana Sajikumar and Sajikumar S

This study was undertaken to establish comprehensive pharmacognostic standards, microscopic characteristics, and the thin-layer chromatographic (TLC) profile of the ingredients used in Amruthotharam Kashayam, a traditional polyherbal formulation. To authenticate the formulation and assess its suitability for medicinal use, key physicochemical parameters such as total ash, acid-insoluble ash, and water and alcohol extractive values were determined for the whole plant materials. Detailed macroscopic and microscopic evaluations, as well as TLC analyses of both methanolic and hydro-methanolic extracts, were conducted. Anatomical studies focused on the stem, rhizome, and fruit rind. The pharmacognostic, microscopic, and TLC profiles generated in this study provide crucial baseline data for the identification, standardization, and quality control of the ingredients in Amruthotharam Kashayam. These findings substantiate its traditional therapeutic use and offer a foundation for future phytochemical and pharmacological investigations.

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