Vol. 2, Issue 2 (2013)
In vitro Germination of Ovules and Plant Regeneration for Cloning and Conservation of Rauwolfia serpentina
Author(s): Devla, Md. Shahnawaz, Raageeva Bimal
Abstract: In an era of species extinction, conservation of biological diversity has assumed global importance. Consequently, biodiversity has become a hotly debated topic in the recent years. The Red Data Books of various regions exemplified the magnitude of vanishing genetic diversity of the biosphere and the rates of species extinction that may threaten mankind. Rauwolfia serpentina, a highly significant medicinal plant is one of the 20 endangered plants for which National Gene Bank at NBPGR, IARI, New Delhi has been standardizing protocol for in vitro conservation (Annual report 1997-1998, Department of Biotechnology, Government of India, New Delhi, p.39). In the present paper, a simple biotechnological protocol for plant regeneration from green fertilized ovary pieces cultured on MS basal medium supplemented with CW(10%) both in culture room at 250C ± 30C and at room temperature has been reported which may be helpful in developing tissue culture nursery for supply of plantlets to herbal farmers. The tissue culture protocol may be used for conservation of R. serpentina.
In vitro plant regeneration in Rauwolfia serpentina. Fig.1-2 - Photographs of R. serpentia showing flowers, green and black fruits. Fig. 3 – Photograph shows transversely cut green ovary pieces cultured on MS medium supplemented with CW (10% v/v) showing callus and a tiny shoot. Fig. 4 – Photograph shows white callus proliferation from cut ovary pieces cultured on MS+CW (10% v/v). Figs.5-6 – photographs showing callus proliferation and shoot as well as plant regeneration from cut ovary pieces cultured on MS+CW (10% v/v). Fig. 7 - R. sepentina plant growing in pot.
How to cite this article:
Devla, Md. Shahnawaz, Raageeva Bimal. In vitro Germination of Ovules and Plant Regeneration for Cloning and Conservation of Rauwolfia serpentina. J Pharmacogn Phytochem 2013;2(2):193-196.