Author(s):

Md. Jakir Hossain, Laila Khaleda, Mohammad Al-Forkan

Abstract: The current situation of medicinal plants and increasing demand of plant derived drugs suggest an immediate need to conserve our medicinal plant resources. The present study attempted to develop in vitro micropropagation protocol for the important medicinal plant Achyranthes bidentata Blume. Shoot tip and nodal segment of healthy plants from natural condition were used as explants for direct organogenesis. Varying concentrations of auxins and cytokinins both alone and in combination were used. Nodal segment explants produced best response in terms of shoot induction percentage and maximum number of shoot than shoot tip.Single cytokinin media was far better than combined effect of cytokinins and auxins. 5.0 mgL-1 and 4.5 mgL-1 of BAP (6-Benzyl amino purine) produced the highest number of shoot which was 10.5 and 10.2, respectively from nodal segment explants whereas the same media produced 9.8 and 9.2 shoot from shoot tip. For root induction and root development, IBA (Indole-3-butyric acid) in full strength MS media was better than IBA in ½ MS (Murashige and Skoog) media. Among different concentrations of full and ½ strength MS media tested, 1.5 mgL-1 of IBA in full strength MS media proved to be the best in terms of root induction (97.78 %) and root number (15.8). Almost 80 % of in vitro grown shootlets were established and acclimatized in natural soil.

Pages: 06-13  |  2549 Views  338 Downloads

Download (353KB)