Vol. 2, Issue 4 (2013)
Simultaneous Extraction, determination and analysis of Adenosine, Cordycepin and other derivatives of Cordyceps sinensis of Nepal by new validated HPLC method
Author(s): Mr. Hemanth Kumar 1*, M. Spandana
Abstract: Cordyceps sinensis, a well-known and valued traditional Chinese medicine, is also called DongChongXiaCao (winter worm summer grass) in Chinese. Parasitic Cordyceps fungi, such as Cordyceps sinensis, are a parasitic complex of fungus and caterpillar, which has been used for medicinal purposes for centuries particularly in China, Japan and other Asian countries. It is commonly used to replenish the kidney and soothe the lung for the treatment of fatigue, night sweating, hyposexualities, hyperglycemia, hyperlipidemia, asthenia after severe illness, respiratory disease, renal dysfunction and renal failure, arrhythmias and other heart disease, and liver disease. Therefore, quality control of C. sinensis and its products is very important to ensure their safety and efficacy. A new HPLC method was developed to determine the content of the Cordycepin and Adenosine in caterpillar fungus. HPLC method was performed on a protoSIL C18 reversed-phase column (250×4.6mm, 5μm). All of the reference substances and sample were separated with the mobile phase of methanol: water (20:80) under isocratic elution for 25min, flow rate was 1.0mL/min, the detection wavelength was 254nm, and the column temperature was 30 ℃.The content of the Cordycepin and Adenosine in the standardized extract of caterpillar fruiting body of fungus were 0.288 mg/g and 0.346 mg/g. The average recovery rate were about 101.1 % ( n=9, RSD=1.33%) in the caterpillar fruiting body. This developed method, which is simple and with high sensitivity as well as selectivity, can be used for quality evaluation of the Cordyceps.
How to cite this article:
Mr. Hemanth Kumar 1*, M. Spandana. Simultaneous Extraction, determination and analysis of Adenosine, Cordycepin and other derivatives of Cordyceps sinensis of Nepal by new validated HPLC method. J Pharmacogn Phytochem 2013;2(4):43-45.