Vol. 2, Issue 6 (2014)
Evaluation of antioxidant activities and total phenolic content of methanolic extract of Rhodiola heterodanta roots
Author(s): Raj Kumar, Somen Acharya, Sharad Rai, OP Chaurasia, RB Srivastava
Abstract: TRhodiola heterodanta belong to the stone crop family Crassulaceae, is an important medicinal plant and food crop in Trans-Himalayan cold desert. It is a well-known medicinal plant in the Amchi system of medicine (Tibetan system of medicine) being used in various ailments like anti-stress, anticancer, radio-protective, adaptogens, anti-inflammatory agent etc. Methanolic extract of R. heterodanta root was investigated in the present study to quantify total Phenolic content as well as anti-oxidant activity. Determination of total phenolic content of the methanol extract of R. heterodanta was achieved using Folin–Ciocalteau reagent. It was found to be 179.42±.63 mg GAE/g plant extract. The DPPH activity of the extract (0.05–0.5 mg/ml) was increased in a dose dependent manner, which was found in the range of (25.38-73.69%) as compared to ascorbic acid (31.48 –77.17%). The IC50 values of methanol extract in DPPH radical, hydroxyl radical, nitric oxide radical and superoxide radical were obtained to be 0.28, 0.27, 0.28, 0.26 mg/ml, respectively. However, the IC50 values for the standard ascorbic acid were noted to be 0.21, 0.24, 0.22., 0.19 mg/ml, respectively. These results clearly indicate that R. heterodanta is effective against free radical mediated diseases and also helpful to draw special attention for further studies.
How to cite this article:
Raj Kumar, Somen Acharya, Sharad Rai, OP Chaurasia, RB Srivastava. Evaluation of antioxidant activities and total phenolic content of methanolic extract of Rhodiola heterodanta roots. J Pharmacogn Phytochem 2014;2(6):189-194.