Vol. 4, Issue 3 (2015)
Evaluation of Antibacterial Activity of Selected Libyan Medicinal Plants against Staphylococcus aureus and Bacillus subtilis
Author(s): Abdoulraouf A Habibi, Sadek A Zubek, Abdulkareem Elbaz, Sabry A El-Khodery, Hamdy Y Osman, Emad M Bennour
Abstract: Medicinal plants could represent an alternative promising antimicrobial agents. The aim of this study was to evaluate the antibacterial activity of Zizyphus Vulgaris, Laurus nobilis, Thymus capitatus, Cistus salvifolius, Arbutus pavarii, Rhus tripartata and Pistacia atlantica against reference strains of Staphyloccocus aureus and Bacillus subtilis. For this purpose, extract of leaves was obtained by 96% chloroform, 95% ethanol or petroleum ether. Antibacterial activity and minimum inhibitory concentration (MIC) for different extracts of each plant was evaluated. Zizyphus Vulgaris, Cistus salvifolius, Thymus capitatus and Arbutus pavarii were the most effective, as the values of inhibition zones were 20 ±2.5 mm, 18 ±2.1mm, 14 ±2.2mm and 15 ±2.0mm for Staphyloccocus aureus, and 10 ±2.1, 14.0 ±1.9, 15 ±2.0, and 15 ±1.7mm for Bacillus subtilis, respectively. However, Pistacia atlantica and Laurus nobilis were less effective against Staphyloccocus aureus and Pistacia atlantica and Rhus tripartata were less effective against Bacillus subtilis. Cistus salvifolius extract caused an inhibition of Staphyloccocus aureus growth at 25 mg/ml while the extract of Thymus capitatus, Arbutus pavarii, Rhus tripartata and Pistacia atlantica caused an inhibition at 50 mg/ml. However, the minimal inhibitory concentration was 200mg/ml for other plant extracts. The minimal inhibitory concentration for Bacillus subtilis was 50 mg/ml for Thymus capitatus, Arbutus pavarii and Pistacia atlantica extract, 100 mg/ml for Cistus salvifolius and 200 mg/ml for other tested plant extracts. Petroleum ether extract of Zizyphus Vulgaris was the most effective causing an inhibition zone of 14mm on Staphylococcus aureus growth. However, petroleum ether extract of Cistus salvifolius was the most effective antimicrobial agent against B. subtilis growth. Zizyphus Vulgaris extract was effective at 100 mg/ml, and Cistus salvifolius was effective at 200 mg/ml. Chloroform extract of Rhus tripartata and Thymus capitatus were effective against Staphylococcus aureus at100 and 200 mg/ml respectively. The results of the present study indicate that ethanolic extract of Cistus salvifolius, Arbutus pavarii and Rhus tripartata has an effective and potent antibacterial activity.
How to cite this article:
Abdoulraouf A Habibi, Sadek A Zubek, Abdulkareem Elbaz, Sabry A El-Khodery, Hamdy Y Osman, Emad M Bennour. Evaluation of Antibacterial Activity of Selected Libyan Medicinal Plants against Staphylococcus aureus and Bacillus subtilis. J Pharmacogn Phytochem 2015;4(3):16-20.