Vol. 5, Issue 2 (2016)
Evaluation of phytochemicals and in vitro antioxidant activity of Ramalina pacifica and Roccella montagnei
Author(s): Smitha KC, Rajkumar H Garampalli
Abstract: The experiment conducted to record the phytochemicals present and antioxidant activities of two medical medicinal lichens Ramalina pacifica and Roccella montagnei. The extracts were obtained in different solvents of chloroform, ethyl acetate, ethanol and methanol. Phytochemical analysis revealed the presence of secondary metabolites namely, alkaloids, carbohydrates, saponins, phytosterols, steroids, phenols, tannins, flavanoids, and proteins. Phytosterols were present in high concentration in all the extracts compare to other secondary metabolites. The antioxidant activity was determined by DPPH radical scavenging activity, reducing power assay (FRAP) and the total phenolic content of all extracts. The ethanol extract of Ramalina pacifica was found to be more effective in free radical scavenging activity which showed 76.72±0.19 and the reducing power which showed 72.38±0.13. Roccella montagnei did not show any significant activity when compare to the Ramalina pacifica. The in vitro studies clearly showed that the ethanol extract of the Ramalina pacifica may be a good source of natural antioxidants.
Antioxidant activity of the lichen extracts 100µg ml
How to cite this article:
Smitha KC, Rajkumar H Garampalli. Evaluation of phytochemicals and in vitro antioxidant activity of Ramalina pacifica and Roccella montagnei. J Pharmacogn Phytochem 2016;5(2):270-274.