Author(s):

Sweety Majumder and Md. Mahbubur Rahman

Abstract: An efficient and reliable micropopagation technique for Clausena heptaphylla (Roxb.) was developed using different explants and media. Shoot apex and nodal explants of field grown plants were aseptically cultured on Murashige and Skoog (MS) medium supplemented with different concentration and combinations of four plant growth regulators (PGRs,) namely IAA, NAA, BAP and Kn. Direct multiple shoot buds developed within 20 days in all explants in most media tested. The highest number of multiple shoot buds (4.20 ± 0.18) was obtain from nodal explants on MS medium containing 2.0 mg/l BAP+1.0 mg/l IAA. Multiple shoot buds underwent rapid elongation (5.60 ± 0.28) on elongation medium took place on MS media fortified with 1.5 mg/lBAP+1.0 mg/l IAA. Elongated shoot buds produced strong and stout roots in rooting media. Half strength MS media supplemented with1.0 mg/l IBA + 0.5 mg/l IAA was better for induction and proliferation (3.56 ± 0.10) of roots. 91% of plantlets were successfully acclimatized to ex vitro condition, exhibiting a normal development.

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