To evaluate the anti-inflammatory activity of ethyl acetate extracts and pure molecules of roots of M. malabaricum
, A. serpyllifolia
, and L. aspera
Materials and Methods: Edema was induced by injecting subcutaneously into the sub plantar tissue of the left hind paw of each Wistar rat (150 to 200 g [N=30]), 0.1 mL of 1% carrageenan suspension in saline. Rats were treated with drug vehicle (1% Sodium CMC) and served as normal control and those treated with ibuprofen as standard. Ethyl acetate extracts of M. malabaricum, A. serpyllifolia, L. aspera and pure molecules MM-1, AS-1, LA-1 obtained from ethyl acetate extract were tested for carrageenan induced paw edema. The drug effects were estimated by comparing the maximal edema response during 6 h and the total edema response as the area under the time course curve.
Results: Results showed that the ibuprofen (62.23 ±1.88), crude extract of M. malabaricum (48.55 ± 1.27, 56.52 ± 0.38 and 59.92 ± 0.78), A. serpyllifolia (40.1 ± 2.16, 48.25 ± 1.65 and 55.42 ± 1.91) and L. aspera (41.68 ± 1.08, 54.74 ± 0.88 and 59.68 ± 0.79) significantly inhibited the maximal edema response during the 6 h of the carrageenan-induced rat paw acute inflammation. The total edema response (AUC) was inhibited by 71.6 ± 1.99, 62.31 ± 2.73, and 63.21 ± 1.61, 59.2 ± 1.96, respectively over 6 h.
Conclusion/Future directions: The results suggested that the standard drug Ibuprofen significantly inhibited paw edema, whereas the ethyl acetate extract of M. malabaricum, A. serpyllifolia and L. aspera produced significant and dose dependent effect in reducing paw edema at the 2 phases of inflammation when compared to the drug vehicle treated group.