Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

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Journal of Pharmacognosy and Phytochemistry

Vol. 5, Issue 6 (2016)

Extraction, isolation and characterization of Pyrrolizidine Alkaloids present in Senecio vulgaris Linn grown in Iraq

Author(s): Hussein Kamil Hamid and Enas J Kadhim
Abstract: Objective: Senecio vulgaris Linn. Commonly known as common groundsel, and in Iraq it was called Sheikh Al-Rabee or AL-Kurresa is widely distributed in the middle and south areas of Iraq and there was no systemic study in the literature regarding the phytoconstituents present in the Iraqi Senecio vulgaris L. Hence, the present study was aimed to determine the presence of biologically important secondary metabolites and to study the structures of some isolated pyrrolizidine alkaloids and determine their percent in this plant. Method: The ethanolic extract obtained from the Senecio vulgaris L. was subjected to phytochemical analysis to determine the presence of secondary metabolites according to standard protocols. The alkaloid rich fraction was subjected to preparative thin layer chromatography in order to isolate certain pyrrolizidine alkaloids after authentication the presence of these alkaloids by comparison with their standards. Isolated pyrrolizidine alkaloids were subjected to high performance liquid chromatography analysis and their retention times were compared with the retention times of the standards. Also, Fourier Transform Infrared spectroscopy was used to determine the structure of isolated pyrrolizidine alkaloids. Results: The qualitative analysis revealed the presence of biologically active secondary metabolites like flavonoids, steroids, terpenoids, tannins, saponine, glycosides, and toxic pyrrolizidine alkaloids in the ethanolic extract obtained from the whole plant. Senecionine and seneciphylline/or their geometrical isomer were isolated from alkaloids rich fraction. Conclusion: The results of the present study indicate the presence of many important secondary metabolites present in the ethanolic extract of Iraqi Senecio vulgaris L. which is not determined before in Iraq. Also, this study proved the presence of some pyrrolizidine alkaloids in this plant and their structure was authenticated depending on their standards.
Pages: 28-37  |  2022 Views  181 Downloads
How to cite this article:
Hussein Kamil Hamid, Enas J Kadhim. Extraction, isolation and characterization of Pyrrolizidine Alkaloids present in Senecio vulgaris Linn grown in Iraq. J Pharmacogn Phytochem 2016;5(6):28-37.
Journal of Pharmacognosy and Phytochemistry