Unani System of Medicine (USM) possesses very effective and safe nephroprotective drugs which are useful in renal disorders for a long time by renowned Unani Physicicians. Beekh Kasni (roots of Cichorium intybus
) belongs to the family of asteraceae, which is one such type of drug widely used as a diuretic, nephroprotective and tonic to kidneys. It is also used in kidney diseases of diverse etiology, having different pathological (su-e-mizaj, su-e-tarkeeb or tafarruq-e-ittesal) and symptomatic presentations. The drug Beekh Kasni has not been scientifically studied; therefore it is important to evaluate this clinically important drug scientifically. In the present study, an effort has been made to evaluate the effect of the aqueous and ethanolic extract of Beekh Kasni on Gentamicin induced nephrotoxicity in rats. The test drug was studied for nephroprotective activity.
Material Methods: Nephroprotective activity of the test drug was evaluated in wistar rats. Animals were allocated into 6 groups of 6 animals in each. Group I served as plain control and group II served as negative control in which toxicity was induced by using Gentamicin (80 mg/kg IM once daily for 12 days). Third group served as concurrent test group A was administered with ethanolic extract of Beekh Kasni (3.4 mg/kg orally once daily for 12 days along with Gentamicin 80 mg/kg IM). Fourth group served as concurrent test group B was administered with aqueous extract of Beekh Kasni (3.4 mg/ kg orally once daily for 12 days). Fifth and Sixth group served as pre treated groups were initially administered ethanolic and aqueous extract of Beekh Kasni 3.4mg/kg respectively orally once daily for 15 days and subsequently Gentamicin 80mg/kg IM once daily administered for the next 12 days. On the 13th day the animals were anaesthetized using phenobarbitone at the dose of dose 50mg/kg, blood was collected by cardiac puncture for the estimation of serum cretinine and serum urea (Bayers) using auto analyzer.
Results: Present study revealed that the test drug possesses significant nephroprotective activity. Two important markers of kidney (Serum Urea and Serum Creatinine) were decreased. Nephroprotective activity was further confirmed by findings of histopathological examination of the kidney tissues where the structural integrity was brought to near normal level in both concurrent and pretreated group of animals.
Conclusion: The test drug Beekh Kasni possesses a significantly high level of nephroprotective effect and the findings validated the Unani description of the test drug.