Vol. 6, Issue 3 (2017)
Determination of antioxidant activity and simultaneous RP-HPLC analysis of quercetin, rutin and kaempferol in Citrus macroptera Montruz
Author(s): Khumukcham Nongalleima, T Ajungla and Chingakham B Singh
Abstract: Citrus macroptera Montruz. fruits were collected, its rind was dried and powdered. Three different organic extracts, aqueous (NC1), methanol (NC2) and aqueous methanol (NC3) were prepared. In vitro antioxidant and reverse phase High Performance Liquid Chromatography (RP-HPLC) was done to quantify the quercetin, rutin and kaempferol content in three extracts of Citrus macroptera Montruz. NC1 showed highest antioxidant (DPPH-IC50 87.83 µg/mg, reducing power assay 36.71 µg/mg). Quercetin and kaempferol content was highest in NC2 extract, was quantified as 431.1 µg/mL and 59.50 µg/mL respectively. However rutin content was highest in aqueous methanol extract (260.38 µg/mL). The designed studies indicate remarkable potential of C. macroptera as dietary source of antioxidant and also the presence of important flavonoids. Studies on isolation of lead compound attributing the studied bioactivity is under progress.
How to cite this article:
Khumukcham Nongalleima, T Ajungla, Chingakham B Singh. Determination of antioxidant activity and simultaneous RP-HPLC analysis of quercetin, rutin and kaempferol in Citrus macroptera Montruz. J Pharmacogn Phytochem 2017;6(3):474-478.