Vol. 6, Issue 3 (2017)
Screening of Calophyllum inophyllum L. leaf extracts for cytotoxic, larvicidal, insect repellent and antimicrobial activities
Author(s): Md. Rubel Rana, Nowrin Islam Amin, Abdullah An Naser and Nurul Islam
Abstract: Petroleum ether (Pet. ether) and Chloroform (CHCl3) solvent extracts were prepared from Calophyllum inophyllum L. leaves for testing bioactive potentiality and were assessed against brine shrimp (Artemia salina) for cytotoxicity, against larvae of Culex quinquefasciatus for larvicidal activity; against eggplant aphid (Aphis gossypii) and black bean aphid (Aphis fabae) for repellent activity; and against five pathogenic bacteria and three pathogenic fungi for anti-microbial activity tests. Both the extracts exposed propitious results through brine shrimp lethality assay where Pet. ether extract found most effective with LC50 values 1413.490, 384.766 and 37.562ppm for 12, 18 and 24h of exposure respectively against A. salina. These two extracts showed promising larvicidal activity against the first instar larvae of Culex quinquefasciatus where CHCl3 extract was more active with LC50 values 237.034, 75.492 and 28.783ppm in compare to Pet. ether extract with LC50 values 1854.121, 418.648 and 42.675ppm for 18, 24 and 30h of exposure respectively. In case of repellency test Pet. ether extract found moderately active (P<0.01) against eggplant aphid (Aphis gossypii) and weakly active (P<0.05) against black bean aphid (Aphis fabae) where CHCl3 extract found weakly active (P<0.05) and inactive against eggplant aphid and black bean aphid respectively. The similar extracts were also propitious against five pathogenic bacteria (Agrobacterium sp., Bacillus cereus, Escherichia coli, Shigella dysenteriae and Staphylococcus aureus) at concentrations of 200 and 400μg/disc along with a standard Penicillin 30μg/disc and three pathogenic fungi (Aspergillus niger, Candida sp. and Saccharomyces sp.) at concentrations of 50 and 200μg/disc along with a standard Nystatin 10μg/disc. Between the two extracts Pet. ether showed promising antibacterial and antifungal activities with the zone of inhibition 16 and 20mm; and 6 and 14mm against Sh. dysenteriae bacterium and Candida sp. fungal strains in comparison to standard penicillin (30μg/disc) and standard Nystatin (10μg/disc) with the inhibition zone of 30 and 7mm respectively. The results showed the potential use of C. inophyllum leaf extracts to control aphids, mosquito larvae, bacteria and fungi.
How to cite this article:
Md. Rubel Rana, Nowrin Islam Amin, Abdullah An Naser, Nurul Islam. Screening of Calophyllum inophyllum L. leaf extracts for cytotoxic, larvicidal, insect repellent and antimicrobial activities. J Pharmacogn Phytochem 2017;6(3):612-616.