To evaluate anticancer activity of methanolic extract of Cissus pallida and Cissus vitegenia on MCF 7, HT 29, HeLa, A549 and DLA cells. Also to assess the antioxidant nature of the plant extracts in superoxide and nitrous oxide assay.
Methods: Methanolic extract of both the plants were screened against MCF 7 (Breast cancer cells), HT 29 (Colon cancer cells), HeLa (Cervical carcinoma cells), A549 (Lung adenocarcinoma cells) in in vitro and Daltons Ascites Lymphoma in in vivo model. The effect of the extracts was assessed by percentage inhibition of the cells, changes in the hematological & liver enzymatic levels and antioxidant action of superoxide dismutase and nitrous oxide.
Results: The extract produced cytotoxicity effect on dose dependent manner comparable with that of the standard drugs used. It also reduced the superoxide desmutase and nitrous oxide free radicals tested in vitro. Significant (P<0.001) changes were observed in the haematological parameters and the liver enzymatic levels.
Conclusions: The results obtained were significant in all the cell lines studied and the extract possess antioxidant action shown by the free radical scavenging effect and the protection of cells and prevent the DNA damage were might be due to the phenolic & flavonoids present in the extract.