Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

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Journal of Pharmacognosy and Phytochemistry

Vol. 6, Issue 6 (2017)

Isolation and characterization of Pseudomonas syringae pv. lachrymans from angular leaf spot disease of cucumber (Cucumis sativus L.) and evaluation of its antibiotic sensitivity

Author(s): Md. Firose Hossain, SM Zia Hasan, Zannati Ferdous Zaoti, Md. Faruk Hasan, Uzzal Kumar Acharjee, Md. Asadul Islam, Md. Khalekuzzaman and Biswanath Sikdar
Abstract: The present investigation was conducted to isolate and characterize the causal organism from angular leaf spot disease of cucumber and evaluation of its sensitivity test against some antibiotics and plant extracts. Different biochemical tests were performed to characterize the isolated bacteria from angular leaf spot disease of cucumber. The growth of the isolated bacteria was obtained in 16 hours of incubation at 37 oC and colonies were creamy white in color. The isolated bacteria showed gram negative at gram staining and potassium hydroxide (KOH) test. Isolated bacteria showed positive response in catalase test, indole test, Simmons citrate test and MacConkey agar test while negative results revealed in urease test, motility test and methyl red test. Antibiotics and antimicrobial activity of the isolated bacteria were screened by disc diffusion method. The highest antibiotic activity against the isolated bacteria was found to be 40.0±0.5mm diameter of zone of inhibition by standard erythromycin. The highest antimicrobial activity was found to be 8.3±0.5mm diameter of zone of inhibition by Allium sativum against the isolated bacteria. This study will be helpful for future research to detect and control of this important bacterial disease of cucumber.
Pages: 233-238  |  1114 Views  84 Downloads
How to cite this article:
Md. Firose Hossain, SM Zia Hasan, Zannati Ferdous Zaoti, Md. Faruk Hasan, Uzzal Kumar Acharjee, Md. Asadul Islam, Md. Khalekuzzaman, Biswanath Sikdar. Isolation and characterization of Pseudomonas syringae pv. lachrymans from angular leaf spot disease of cucumber (Cucumis sativus L.) and evaluation of its antibiotic sensitivity. J Pharmacogn Phytochem 2017;6(6):233-238.
Journal of Pharmacognosy and Phytochemistry