Vol. 6, Issue 6 (2017)
Screening of Nanoparticles blended Couroupita guianensis for its antibacterial activity against gram positive and gram negative bacterial pathogens
Author(s): M Gopu, M Gurulakshmi and D Estherlydia
Abstract: Phytochemical analysis, fluorescent character and antibacterial activity of Couroupita guianensis flower extract (petals and stamens) were investigated. The crude extract showed the presence of alkaloids, carbohydrate Flavonoid, Terpenoid and Gum and Mucilage. The solvents used for the extraction of flower were water and methanol. Further, the antibacterial activity of titania nanoparticles were compared with titania nanoparticles blended Couroupita guianensis extract. The in-vitro antibacterial activity was tested by well diffusion method against four bacterial pathogens: Escherichia coli, Staphylococcus aureus, Staphylococcus mutans and Klebsiella pneumonia. The antibacterial results showed methanol extracts of Couroupita guianensis for both petal and stamen showed maximum zone of inhibition – MIC against S. aureus and E. coli, respectively. The methanol extract of Couroupita guianensis blended with TiO2 showed an enhanced phytochemical extract of petal and stamen. MIC was tested at various concentrations for both petals and stamen extracts blended with TiO2. The methanol extract of Couroupita guianensis blended with TiO2 with crude petal and stamen showed higher MIC% against E. coli. Thus based on the inhibitory concentration, the two extract blended with nanoparticles has potent antibacterial activity and can be used for treating drugs resistant human pathogens.
How to cite this article:
M Gopu, M Gurulakshmi, D Estherlydia. Screening of Nanoparticles blended Couroupita guianensis for its antibacterial activity against gram positive and gram negative bacterial pathogens. J Pharmacogn Phytochem 2017;6(6):652-662.