Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

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Journal of Pharmacognosy and Phytochemistry

Vol. 6, Issue 6 (2017)

Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare

Author(s): Suman Tiwari and OP Verma
Abstract: β-glucosidase (EC 3.2.1.21) was extracted from spikes of Hordeum vulgare and was purified using ammonium sulphate fractional precipitation and Sephadex G-25 chromatography. The molecular weight of enzyme was found in the range of 13-85KDa .The enzyme β-glucosidase has optimum pH 5.0 and the optimum temperature was found at 60ºC. Bioethanol was produced from spikes of Hordeum vulgare.
Pages: 1657-1659  |  317 Views  6 Downloads
How to cite this article:
Suman Tiwari, OP Verma. Isolation, partial purification, product formation and characterization of β- glucosidase from spikes of Hordeum vulgare. J Pharmacogn Phytochem 2017;6(6):1657-1659.
Journal of Pharmacognosy and Phytochemistry