Vol. 6, Issue 6 (2017)
Bioassay guided fractionation of antifungal activity of Jatropha curcas
Author(s): Krishnananda Pralhad Ingle, Amit Gulabrao Deshmukh, Dipika Ashokrao Padole, Mahendra Shankarrao Dudhare, Mangesh Pradip Moharil, Vaibhav Chandrakant Khelurkar and Dhiraj Raghunathrao Gangtire
Abstract: Bioactive constituents from plants are an integral component of research and development in the pharmaceutical and agrochemical industry. Plants face many stresses in their life cycle and in the process produce secondary metabolites. These secondary metabolites are not important for the metabolic functions of the plant but help to face many stressful conditions like diseases, pests, etc. Some of these secondary metabolites have capacity to fight microorganisms and thus can be used as antimicrobial agents. The present study has exploited the probability of having any antimicrobial molecules present in the Jatropha curcas. In the present study root, leaf, bark, seed, and seed coat of Jatropha curcas were extracted with methanol using reflux extraction method. The crude methanolic root extract was found to have better antifungal activity (upto 23.1% growth inhibition) against Rhizoctonia. The root extract when fractionated by column chromatography showed upto 72.72 % growth inhibition against Fusarium udum and 41.17% against Rhizoctonia bataticola in toluene fraction 1. It was further screened for HPTLC and FTIR to characterize fraction in functional groups.
How to cite this article:
Krishnananda Pralhad Ingle, Amit Gulabrao Deshmukh, Dipika Ashokrao Padole, Mahendra Shankarrao Dudhare, Mangesh Pradip Moharil, Vaibhav Chandrakant Khelurkar, Dhiraj Raghunathrao Gangtire. Bioassay guided fractionation of antifungal activity of Jatropha curcas. J Pharmacogn Phytochem 2017;6(6):2147-2154.