Vol. 7, Issue 1 (2018)
Antibacterial, brine shrimp lethality assay and GC-MS analysis of Piper longum Linn
Author(s): Ram Lal Shrestha
Abstract: Fruit part of Piper longum Linn. was subjected to extraction of essential oil by hydro distillation in Clevenger apparatus. Methanol was used to extract the dried and powdered plant. The composition of essential oil so collected were determined by GC-MS system and showed the presence of 17 different compounds. The most abundant were germacrene (25.04%), heptadecene (11.71%), heptadecane (9.23%), β-bisabolene (8.36%) and caryophyllene (7.71%). Antibacterial activity of the essential oil of P. longum was studied. Oil exhibited moderate antibacterial activity against Klebsiella pneumoniae. The LD50 of the sample was found to be 251.1μg/mL.
How to cite this article:
Ram Lal Shrestha. Antibacterial, brine shrimp lethality assay and GC-MS analysis of Piper longum Linn. J Pharmacogn Phytochem 2018;7(1):800-802.