Vol. 7, Issue 1 (2018)
Varietal identification in French bean through banding pattern of seed storage protein and its change due to storage
Author(s): AK Hembram, Venkata Satish Kuchi and AK Basu
Abstract: Seed storage protein of five French bean varieties viz. Sonali, Abhay, Falguni, Mandulal and Selection-9 were extracted from both harvest fresh and one year old seed stored in desiccator. SDS-PAGE of storage protein of all the varieties were analyzed critically and tried to study the variation in banding pattern due to storage. A total number of 32 bands with different Rm values were noticed for harvest fresh seeds of five varieties out of which a minimum of 10 bands were noticed for Selection-9, 11 for Sonali, 12 for Falguni, 14 for Abhay and 16 for Mandulal. Comparison of banding pattern between fresh and old seeds of individual varieties indicate a varying scenario: for Sonali, band number 4, 15, 18 and 28 were disappeared in old seeds and instead 3 different bands newly appeared with Rm values 0.18, 0.56 and 0.85; for Abhay 2 bands with Rm values 0.23 and 0.94 were disappeared from old seeds; for Falguni also 2 bands with different Rm values (0.65 and0.81) could not be identified in old seeds; for Mandulal, total 14 bands were noted for old seeds, of which 4 bands from fresh seed (Rm values 0.43, 0.48, 0.65 and 0.82) disappeared in old seeds and additionally 2 bands with Rm values 0.66 and 0.81 newly appeared in old seeds, and it is interesting to note that band numbers remain same in both fresh and old seeds of Selection-9 with a very minute change in width of band number 8 (Rm value 0.34). Therefore band number, their position, thickness as well as disappearance/ new appearance clearly help in identification of those five varieties under consideration.
How to cite this article:
AK Hembram, Venkata Satish Kuchi, AK Basu. Varietal identification in French bean through banding pattern of seed storage protein and its change due to storage. J Pharmacogn Phytochem 2018;7(1):1972-1975.