Author(s):

Sweta Mishra and Yuvraj K Vala

Abstract: An efficient and reproducible plant regeneration protocol was developed from petiole and axillary bud explants derived from physiologically mature trees of Simarouba glauca DC, a promising medicinal tree that has potential to offer an alternative source of edible oil. This is the first ever report of plant regeneration protocol for mature trees of Simarouba glauca DC. PVP (1.5 g/l), ascorbic acid (100 mg/l) and citric acid (25 mg/l) was used to effectively counter the phenolics released from the explants of the mature tree. Petiole explants appeared to have better regeneration capacity than axillary bud explants. The petiole explants produced 88.47 % embryogenic calli on MS + BA (2.5 mg/l) + Kinetin (1.5 mg/l) + IBA (0.5 mg/l) + NAA (0.1 mg/l). The calli produced were green and friable. The maximum number of shoots (09.63±0.11) with a shoot length of 05.41±0.12 cm were produced from theses embryogenic calli derived from the petiole explants on the MS medium supplemented with 2.0 mg/l BA + 1.0 mg/l 2ip + 0.3 mg/l GA₃. The petiole explants showed a shoot induction per cent of 92.34±0.12 which was higher than that produced from the axillary bud explants. Profuse rooting was obtained from the shoots regenerated from the petiole explants on the MS medium supplemented with IBA (0.1 mg/l) + NAA (0.5 mg/l) having a root induction response of 92.50±0.06 % with the highest number of roots (06.72±0.13) and root length (05.74±0.16 cm). The survival percentage of the plants in the field after acclimatization was 85.5%.

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