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Journal of Pharmacognosy and Phytochemistry
Vol. 7, Issue 2 (2018)
Development and validation of RP-HPLC method for simultaneous determination of paraben preservatives in pharmaceutical liquid dosage
Author(s): Neha Tiwari, UVS Teotia and Yogendra Singh
Abstract: A reversed phase HPLC method has been developed that allows the simultaneous estimation of the methyl paraben (MP) and propyl paraben (PP) in pharmaceutical liquid dosage form. The mobile phase was filtered and degassed mixture of buffer (pH 2.0) and acetonitrile (68: 32). The detector wavelength was set at 205 nm and flow rate was 1.0 mL/min. Under these conditions, separation of the two components was achieved in less than 10 min. Validation parameters allow to consider the method adequate. The method was successfully used for estimating both paraben preservatives in pharmaceutical liquid formulation.
How to cite this article:
Neha Tiwari, UVS Teotia, Yogendra Singh. Development and validation of RP-HPLC method for simultaneous determination of paraben preservatives in pharmaceutical liquid dosage. J Pharmacogn Phytochem 2018;7(2):1243-1245.