The aim of this study was to investigate the antibacterial activity and in vitro
anti-thrombolytic activity of Typha angustifolia
Methods: The thrombolytic activity was screened by in-vitro clot lysis model. In this model the blood sample (500μl/tube) from human volunteer has been collected in micro centrifuge tubes. After incubation the standard drug and different dilutions of test drug were added whereas water is taken as control. Percentage of Clot lysis was calculated on the basis of the weight difference of micro centrifuge tubes obtained before and after clot lysis. The antibacterial properties were determined by agar well diffusion method against S. aureus, B. subtilis, K. pneumoniae, E. coli, P. aerugenosa.
Result: Solvent extracts of T. angustifolia significance clot lysis activity with reference of Streptokinase as standard. Ethanol and Acetone showed strong antibacterial activity against S. aureus, E. coli and P. aerugenosa (18mm), Methanol showed moderate antibacterial activity against S. aureus, B. subtilis and P. aerugenosa (17mm). Aqueous solution showed antibacterial activity against B. subtilis, P. aerugenosa, S. aureous, E. coli (16mm). Acetone showed moderate antibacterial activity against K.pneumoniae. Ethanol and Methanol showed mild antibacterial activity against K. pneumonia and E. coli (15mm). Aqueous solution showed mild antibacterial activity against K. pneumoniae.
Conclusion: The present investigation revealed that Typha angustifolia fibre extracts Possesses positive thrombolytic properties that could lysis blood clots. Further studies using in vivo models are required to carry out and establish the effectiveness and pharmacological rationale for the use of Typha angustifolia fibre extracts as a thrombolytic drug ethanol and acetone extract of T. angustifolia possess strong anti-bacterial activity against bacterial pathogen.