One of the ingredients of environmental pollution is microbicides, bactericide, fungicides etc. and their overuse lead to antimicrobial resistance. This research analyses the use of phytoconstituents as fungicides, on
, a fungus using different percentage of solvents-extracts.
The extracted solvent-extracts of Withania sominifera, Azadirachta indica
and Asparagus racemosus using different percentages of different solvents were used to test the inhibition of the pathogen at the rate of 1.73 spores/ml as spore suspension with the help of Plate reader / Spectrophotometer. For confirmation, the culture of the result was done using suitable culture media.
Result: The percentage-extraction of phytoconstituents differs with different solvents and their aqueous mixture. High percentage of extraction of phytoconstituents (mg/gm) of Withania somnifera leaves was obtained in 80% acetone and n-hexane and high concentration of extraction of phytoconstituents (mole/liter) of Azadirachta indica leaves was obtained using aqueous mixture of the acetone. 75% and 100% methanol and acetone extracts inhibited Alternaria solani by 83%, 96%, 92%and 95% respectively after 2 hours of incubation. 80% of acetone and n-hexane extracts of Withania somnifera inhibited Alternaria solani by 85% and 84% respectively.
Conclusion: Aqueous mixture of acetone and ethanol may be the better solvents for extraction of phytoconstituents to obtain maximum antifungal activity (here on Alternaria solani by Azadirachta indica and Withania somnifera) and therefore, phytoconstituents can be used as an alternate form of fungicides.