Author(s):

Priyanka and Geeta Goudar

Abstract: The present study was conducted on in-vitro screening of fluorescent pseudomonads against Colletotrichum truncatum of soybean. Out of 62 native fluorescent pseudomonads isolates from soybean rhizosphere, 38 isolates showed antagonistic activity against Colletotrichum truncatum under dual plate inoculation technique. The zone of inhibition ranges from 2.20 to 6.35 cm with per cent inhibition of 24.44% to 74.36%. Among 38 isolates, five isolates viz, BFP22, DFP62, DFP56, BFP42 and DFP54 were found potent with 45. 56 - 70.37 per cent inhibition of mycelial growth against C. truncatum. Five isolates were further tested for their growth promotional properties like, HCN production, siderophore production, IAA and GA production. The isolate DFP48 showed highest IAA production of (28.89 mg/25 ml), DFP54 recorded highest GA production of 18.52 mg/25ml, ten isolates exhibited strong HCN production and DFP54 showed highest siderophore production (36.37mm zone of clearance on CAS media).

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