Vol. 9, Issue 4 (2020)
Biochemical constituents in transgenic mulberry expressing barley HVA1, tobacco osmotin and BCH1 (β-Carotene Hydroxylase) genes
Author(s): SR Manjunatha, Manjunath Gowda, Nataraja Karaba, KC Narayanaswamy and KS Jagadish
Abstract: Mulberry is a perennial tree crop, trait improvement through traditional breeding is cumbersome and time consuming. Recently, mulberry transgenic plants expressing Arabidopsis gene, AtSHN1 to improve post-harvest water loss and impart stress tolerance has been developed. Mulberry is rich sources of protein, carbohydrate, carotenoids, lipids, ascorbic acid, anthocyanins etc. Hence the current investigation was undertaken to study the biochemical constituents in leaves of transgenic mulberry lines. Biochemical analysis revealed that transgenic mulberry lines showed significant difference for chlorophyll a content with maximum being in non-transgenic genotype V1 (0.84 mg/g FW) followed by transgenic lines, ST series (0.87 mg/g FW) and BT series (0.81 mg/g FW). Similarly, Highest chlorophyll b content was observed in non-transgenic mulberry line V1 (0.83 mg/g FW) followed by transgenic lines ST series (0.78 mg/g FW), MT series (0.74 mg/g FW) and BT series (0.70mg/g FW). For the total chlorophyll content, the experiment revealed that all six treatments were significantly different. Among six treatments, V1 was found to be having highest total chlorophyll content (1.66 mg/g FW). The maximum protein content of 14.25 mg/g was noticed in transgenic lines, MT series followed by RD series (14.00 mg/g) wherein non-transgenic type the content was around 11.50 mg/g tissue. The highest carbohydrate content (0.28 mg/g) was recorded in ST series followed by V1 series which recorded 0.27 mg/g of carbohydrate content and RD series with 0.24 mg/g of carbohydrates.
How to cite this article:
SR Manjunatha, Manjunath Gowda, Nataraja Karaba, KC Narayanaswamy, KS Jagadish. Biochemical constituents in transgenic mulberry expressing barley HVA1, tobacco osmotin and BCH1 (β-Carotene Hydroxylase) genes. J Pharmacogn Phytochem 2020;9(4):238-241.