Vol. 9, Issue 4 (2020)
Acute and sub-acute (28-Day) oral toxicity studies of aqueous extract of secamone afzelii leaves in wistar rats
Author(s): Koné Djoudori Serge, Gnahoue Goueh, Tra bi irié otis, Bamba Abou, Kouakou Koffi Roger and Yapi Houphouet Félix
Abstract: Aim of the study: The present study was carried out to evaluate the acute and subacute toxicity of the aqueous extract of Secamone afzelii (AESA) Materials and Methods: Acute toxicity study was conducted in rats by using OECD 423 guidelines whereas sub-acute toxicity study was carried out in rats by using OECD 407 guidelines. In the acute toxicity study, rats were orally administered with a single dose of 2000 mg/kg and then observed individually for the first four hours, then over a period of 24 hours and at least once daily for 14 days. In the subacute toxicity studies, AESA was given orally at doses of 200 mg/kg, 400 mg/kg and 800 mg/kg body weight daily for 28 days to male and female rats respectively. General behavior, adverse effects and mortality were observed throughout the experimental period. Food intake, water intake, body weight, organ weight, hematological and biochemical parameters were evaluated. Results: The limit dose of 2000 mg/kg did not cause any mortality or signs of acute toxicity in the rats tested during the observation period. In sub-acute toxicity tests, the results did not show any treatment related abnormalities in terms of hematological but showed biochemical parameters abnormalities. There was a significant increase (p<0, 05) of total protein in male rats treated with 200 mg/kg of AESA compared to control group. The male rats treated with 800 mg/kg of AESA, also indicated a significant elevation of Creatinine concentration (p<0, 01) compared to control group. There were no significant differences in body weight and organ weight between the control and treated groups. Conclusion: These results concluded that the AESA did not cause any mortality and signs of toxicity in rats (acute toxicity study). The oral lethal dose of aqueous extract of Secamone afzelii is more than 2000 mg/kg. In the sub-acute toxicity, any mortality and signs of toxicity in female rats. But, in male rats, subacute study showed that Secamone afzelii at the high dose (800 mg/kg per day for 28 days) may cause renal dysfunction.
How to cite this article:
Koné Djoudori Serge, Gnahoue Goueh, Tra bi irié otis, Bamba Abou, Kouakou Koffi Roger, Yapi Houphouet Félix. Acute and sub-acute (28-Day) oral toxicity studies of aqueous extract of secamone afzelii leaves in wistar rats. J Pharmacogn Phytochem 2020;9(4):60-64.