Vol. 10, Issue 1 (2021)
Phytochemical analysis and antioxidant activity of Trigonella foenum-graecum seeds
Author(s): Naveen Kumar, AH Ahmad, SP Singh, Disha Pant, Amit Prasad and SK Rastogi
Abstract: Trigonella foenum graecum (Fenugreek) (Family: Leguminosae) is used as a traditional medicine in treating various diseases due to its antiinflammatory, antidiabetic and antilipidemic properties. In our study, aqueous extract of seeds were subjected for in vitro antioxidant activity by 1- diphenylpicryl- hydrazyl radical (DPPH), and ABTS radical cation assay. Total phenol and total flavonoid content were estimated. It was observed that free radicals were scavenged by the extract in a dose dependent manner. The content of total phenolics (expressed as mg of gallic acid equivalents/gm extract) and total flavonoids (expressed as mg of quercetin equivalent/gm extract) were determined along with antioxidant enzymes. The aqueous extract of fenugreek exhibited potent DPPH and ABTS radical scavenging activity with IC50 values of 62.67 μg/mL and 71.44 μg/mL, respectively. Based on the results it can be concluded that aqueous seed extract of fenugreek seeds may have potential antioxidant effects against several oxidants generated in the body.
How to cite this article:
Naveen Kumar, AH Ahmad, SP Singh, Disha Pant, Amit Prasad, SK Rastogi. Phytochemical analysis and antioxidant activity of Trigonella foenum-graecum seeds. J Pharmacogn Phytochem 2021;10(1):23-26.