Author(s):

Rifat Ara Rima, Shoriful Islam Sagor and Adeeba Anjum

Abstract: In the present study, antioxidant potential of the crude methanol extract along with its n-hexane, chloroform, ethyl acetate and aqueous soluble fractions of the roots ofCoffeabenghalensisgrowing in Bangladesh was assessed by usingDPPHscavenging assay, reducing power, hydroxyl radical scavenging assay and total antioxidant capacity.In DPPH scavenging assay the IC₅₀ value of the ethyl acetate soluble fraction was found to be 47.56 µg/ml while the reference standard ascorbic acid was 1.42 µg/ml. The reducing power showed good linear relations between the standard ascorbic acid and all the test samples. Higher hydroxyl radical activity was shown by the n-hexane soluble fraction with IC₅₀ value 58.58 µg/mlwhereas the standard catechin was 89.61 µg/ml. The extracts showed significant activities in total the antioxidant assaycompared to the reference standard in a dose dependent manner. Also total phenolic and flavonoid content was evaluated which exhibited remarkable values.

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