Journal of Pharmacognosy and Phytochemistry
Vol. 1, Issue 6 (2013)
Phytosteroids from tissue culture of Allium cepa L. and Trachyspermum ammi S prague.
Dr. Pratibha Chaturvedi 1*, Dr Pushpa Khanna 2 and Dr. Abhay Chowdhary 1
Production of secondary metabolites by cultured cells provides a particularly important benefit to manipulate and improve the production of desired compounds; thus biotechnological approaches to increase the concentrations of the metabolites are discussed. Present study deals with the production, isolation and identification of phytosterols from tissue culture of Allium cepa and from plant parts and tissue culture of Trachyspermum ammi. Steroidal analysis of plant parts showed the maximum amount of stigmasterol (0.240 mg/gdw) which was comparatively little less than that of the amount of Î²- sitosterol (0.295 mg/gdw) in the seeds of T. ammi . The maximum amount of stigmasterol was present in four weeks old tissue of T. ammi (0.249 mg/gdw) whereas the highest content of Î²- sitosterol was observed in six weeks old tissue of A. cepa (0.315 mg/gdw) However, lanosterol, was present only in the tissue of A. cepa which was maximum in six weeks old tissue (0.039 mg/gdw).
Pages: 42-48 | 1568 Views 25 Downloads
Dr. Pratibha Chaturvedi 1*, Dr Pushpa Khanna 2 and Dr. Abhay Chowdhary 1. Phytosteroids from tissue culture of Allium cepa L. and Trachyspermum ammi S prague.. J Pharmacogn Phytochem 2013;1(6):42-48.