Journal of Pharmacognosy and Phytochemistry
Vol. 4, Issue 1 (2015)
Comparative extraction methods, fluorescence, qualitative and quantitative evaluation of Ammi majus seed extracts
Aveen Nozad Adham
Ammi majus belong to the family Apiaceae that provide a huge number of compounds with important medicinal activities. The present investigation is aimed to compare the percentage value of Ammi majus extracts by using different solvent and extraction methods, to do fluorescence analysis and phytochemical screening (qualitative and quantitative) for the important natural product groups in Ammi majus seed. The highest percentage extract was yielded from seed by using ordinary reflex methods and n-hexane 12.74%. Phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins, phenolic acids, terpenoids, carbohydrate, coumarin and quinone in all fractions and presence of cardioactive glycoside, saponin, amino acid, carbohydrate, quinones in the seed has not been reported before. Presence of flavonoids estimated to be 288.611Âµg quercetin/gm in seed was determined in high quantity in ethanol 80% extract and tannins content 216.66 Âµg gallic acid/gm in low quantity. The fluorescent characteristics of seed powder with various chemical reagents were noted under visible and UV light.
Pages: 41-44 | 1737 Views 71 Downloads
Aveen Nozad Adham. Comparative extraction methods, fluorescence, qualitative and quantitative evaluation of Ammi majus seed extracts. J Pharmacogn Phytochem 2015;4(1):41-44.