Journal of Pharmacognosy and Phytochemistry
Vol. 6, Issue 5 (2017)
HPLC-DAD-MS method for Simultaneous quantitation of flavonoids in Hypericum formosanum and antiglycation activity
Ho-Shin Huang and Ean-Tun Liaw
(HF) is widely used as traditional and folklore medicine in Taiwan. In the present study, ultrahigh performance liquid chromatography equipped with photodiode array detection-mass (UPLCâ€“DAD-MS) method was developed for the separation, identification, and quantification of bioactive flavonoids. The developed method was also validated for accuracy, precision, limit of detection and quantification (LOD and LOQ). In this method, four flavonoids, viz., hyperoside, astiblin, quercitrin, and quercetin, were quantified in linearity range of 10â€“200 (Î¼g/mL) with correlation coefficient of greater than 0.997. A high recovery (87.55â€“92.11%) and good reproducibility was obtained for four flavonoids with the relative standard deviation (RSD) ranging from 1.75-2.26 % (intra-day) and 1.99-2.27% (inter-day). Hence, the proposed method for simultaneous quantification of four bioactive flavonoids in the extract and fractions of H. formosanum
using UPLCâ€“DAD-MS detection under the specified conditions is accurate and validated. Besides that, ethyl acetate (EtOAc) fraction showed the best anti-glycation and its IC50 was observed at 69.5Î¼g/mL.
Pages: 854-858 | 923 Views 94 Downloads
Ho-Shin Huang and Ean-Tun Liaw. HPLC-DAD-MS method for Simultaneous quantitation of flavonoids in Hypericum formosanum and antiglycation activity. J Pharmacogn Phytochem 2017;6(5):854-858.