Author(s):

Prerit Kumar Singh, RP Diwakar and Aprajita Johri

Abstract: The purpose of this study was to investigate sub-clinical mastitis in dairy farm and prevalence of <em>Staphylococcus aureus </em>with its antibiogram pattern. A total of 80 buffaloes (320 quarters) were investigated for sub-clinical mastitis. Samples positive for sub-clinical mastitis were subjected for isolation and biochemical characterization. Isolates that was confirmed as <em>S. aureus </em>were subjected for antibiotic-susceptibility testing. The overall quarter wise incidence of sub clinical mastitis among 80 buffaloes was 2.4 per cent (90 quarters out of 320 quarters). After biochemical characterization, <em>S. aureus </em>was confirmed in 90 samples. Antibiotic resistance pattern revealed that the highest resistance to the antimicrobials used was to observed in ampicillin (75.00%), followed by chloramphenicol (72.50%), cefotaxime (62.50%), levofloxacin (55.00%) gentamicin (55.00%), cepholexin (50.00%), ceftriaxone and ciprofloxacin (45.00%), penicillin G (40.00%), and tetracycline (42.50 %). This study confirms the importance of <em>S. aureus </em>as a major mastitis causing bacterium and existence of alarming level of resistance to frequently used antibiotic by <em>S. aureus </em>and a potential risk for human health from nearly possible transmission of the <em>S. aureus </em>strains as milk borne pathogen.

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