Vol. 9, Special Issue 5 (2020)
Isolation, identification and pathogenicity of Sclerotinia sclerotiorum causing Sclerotinia rot of chilli
Author(s): Satyadev Prajapati, Shailesh Godika, Naresh Kumar, Lalita Lakhran, Shivam Maurya and Jitendra Sharma
Abstract: Sclerotinia rot caused by Sclerotinia sclerotiorum is one of the most devastating soil borne pathogen, which is threatening the production of chilli crop. In this study associated fungus was isolated on PDA medium and purified by using hyphal tip method from infected chilli plant samples were collected from farmer’s fields. On PDA, uniformly one type growth of fungus colony started without zonation with whitish to gray growth in colour and fungus rapidly covered the entire Petri plate within 72 hours. Fungal mycelium aggregate to form small mycelial tufts which was converted into hard black sclerotia with Oval to irregular shape at the periphery of the Petri plates. Microscopic observation showed, hyphae were hyaline and branched and asci arranged on periphery of ascocarp. Ascus was hyaline, barrel shaped and produced in tightly compact mass with filiform paraphyses at the upper surface of apothecium. Ascospores were elliptical to oval, single celled, hyaline and posses eight numbers in each ascus. Pathogenicity was proved on chilli plants using seed, soil and seed + soil inoculation method. Among the different inoculation methods, seed + soil inoculation recorded maximum disease incidence (72.66%) followed by soil inoculation method (64.00%). On the basis of cultural, morphological and pathogenicity test, isolated fungus was identified as Sclerotinia sclerotiorum.
How to cite this article:
Satyadev Prajapati, Shailesh Godika, Naresh Kumar, Lalita Lakhran, Shivam Maurya and Jitendra Sharma. Isolation, identification and pathogenicity of Sclerotinia sclerotiorum causing Sclerotinia rot of chilli. 2020; 9(5S): 20-23.