Vol. 1, Issue 4 (2012)
Evaluation of Membrane Stabilizing Activity, Total Phenolic Content, Brine Shrimp Lethality Bioassay, Thrombolytic and Antimicrobial Activities of Tagetes patula L.
Author(s): Md. Ruhul Kuddus, Mirza Sonia Alam, Sharmin Reza Chowdhury, Farhana Rumi, Md. Al Amin Sikder, and Mohammad A. Rashid*
Abstract: The methanol extract of leaf of Tagetes patula L. as well as its n-hexane, carbon tetrachloride, chloroform and aqueous soluble partitionates were subjected to screening for total phenolic content, brine shrimp lethality, membrane stabilizing, thrombolytic and antimicrobial activity. The membrane stabilizing activity was assessed by hypotonic solution-and heat-induced methods and was compared with acetyl salicylic acid. In the present studies, the n-hexane soluble fraction demonstrated strong membrane stabilizing activity in both hypotonic solution-and heat-induced methods with 44.48% and 42.68% inhibition of haemolysis, respectively. The total phenolic content was also determined and expressed in gallic acid equivalent. In brine shrimp bioassay, the crude methanol extract of leaf showed strong cytotoxic activity with LC50 value of 8.58 μg/ml compared to that of 0.451 μg/ml exhibited by standard vincristine sulphate. During assay for thrombolytic activity, the n-hexane soluble fraction revealed 43.7% lysis of clot while standard streptokinase and water, used as positive and negative controls, demonstrated 65.8% and 3.62% lysis of clot, respectively. In antimicrobial assay by disc diffusion method, all the samples exhibited moderate to significant antimicrobial activity (zone of inhibition = 9.0-22.0 mm) against all the test organisms. Among all the samples, the carbon tetrachloride soluble fraction displayed strong antimicrobial activity against Escherichia coli (22.0 mm).
How to cite this article:
Md. Ruhul Kuddus, Mirza Sonia Alam, Sharmin Reza Chowdhury, Farhana Rumi, Md. Al Amin Sikder, and Mohammad A. Rashid*. Evaluation of Membrane Stabilizing Activity, Total Phenolic Content, Brine Shrimp Lethality Bioassay, Thrombolytic and Antimicrobial Activities of Tagetes patula L.. Journal of Pharmacognosy and Phytochemistry. 2012; 1(4): 57-62.