Author(s):

Barnali Paul, Prasenjit Mitra,Tanaya Ghosh, Ravinernath Salhan, Takhelmayum Amumachi Singh, Amit Chakrabarti, Sumanta Gupta, Basudeb Basu, Prasanta Kumar Mitra*

Abstract: By different solvent extractions and chromatographic techniques an antibacterial constituent was isolated from leaves of Cassia alata Linn. Infra red spectroscopy, mass spectroscopy and nuclear magnetic resonance studies showed that the isolated compound was chemically 3,4 dihydroxy cinnamic acid. In vitro antibacterial activity of 3,4 dihydroxy cinnamic acid was studied against four Gram-positive and four Gram-negative bacteria using disc diffusion method. Minimum inhibitory concentration (MIC) of 3,4 dihydroxy cinnamic acid was also recorded against those bacteria by serial dilution technique. Kanamycin was used as positive control. Results showed that 3,4 dihydroxy cinnamic acid had antibacterial activity against the tested bacteria.

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