Vol. 4, Issue 2 (2015)
Hepatoprotective and antioxidant activity of Odontonema Cuspidatum (Nees) Kuntze against CCl4-Induced Hepatic Injury in Rats
Author(s): M. S. Refaey, M. A. H. Mustafa, A. M. Mohamed, A. A. Ali
Abstract: Objective: The present investigation deals with evaluation of hepatoprotective and antioxidant activity of total methanol extract and different fractions of Odontonema cuspidatum on the CCl4 induced hepatotoxicity. Material and methods: Albino Wister rats weighing (140±20 g) of either sex were divided into seven groups (n=8). Hepatotoxicity was induced by CCl4 dissolved in olive oil (1:1, 2 ml/kg bw, S.c). Silymarin (100mg/kg) orally was used as standard drug. Test groups received total methanol extract and different fractions (aqueous, n-butanol and n-hexane fractions) of Odontonema cuspidatum aerial partin a dose of 400 mg/kg/day orally along with CCl4. Treatment was given to all the tested groups daily for 5 days. The hepatoprotective effect of Odontonema cuspidatum was evaluated by assessment of biochemical parameters [(AST), (ALT), (ALP) and total bilirubin] and the antioxidant activity in the liver tissue was estimated by measuring the activities of antioxidant enzymes: reduced glutathione (GSH) as well as the level of lipid peroxidation by Malondialdehyde (MDA). Biochemical observations were also supported with histopathological examination of liver section. Results: Odontonema cuspidatum methanol extract (OCME) (400mg/kg) exhibited a highly significant reduction (p<0.05) in AST, ALT, ALP and total bilirubin better than other fractions. Odontonema cuspidatum methanol extract in a dose of 400 mg/kg/d showed highly significant reduction (p<0.05) in MDA and rise in (p<0.05) in GSH better than other fractions. Histopathological examination of the liver denoted marked hepatoprotective effect of the total methanol extract by absence of histopathological lesions, decrease the extent of necrosis and fatty changes when compared to carbon tetrachloride group, while the other fractions showed above moderate effect on CCl4 induced hepatotoxicity in rats. Conclusion: Odontonema cuspidatum showed significant protection against carbon tetrachloride induced liver injury in rats by enhancing the antioxidant defense condition, lessening lipid peroxidation, and conserving against the pathological changes of the liver.
How to cite this article:
M. S. Refaey, M. A. H. Mustafa, A. M. Mohamed, A. A. Ali. Hepatoprotective and antioxidant activity of Odontonema Cuspidatum (Nees) Kuntze against CCl4-Induced Hepatic Injury in Rats. Journal of Pharmacognosy and Phytochemistry. 2015; 4(2): 89-96.