Evaluating Anthelmintic activity of Kandelia candel
and Rhizophora apiculata
extracts by using in vitro assay.
Methods: The serial exhaustive extraction was carried out with a series of solvents: chloroform, ethyl acetate, methanol, ethanol and water with increasing polarity using Soxhlet apparatus. The concentrated and dried extracts were evaluated for anthelmintic activity by employing standard in vitro method (Pheretima Posthuma model).
Results: In vitro anthelmintic study shows that in case of both Kandelia candel and Rhizophora apiculata methanol extract showed higher anthelmintic activity when compared to other solvent extracts.
Conclusion: Results confirm that among tested extracts of both Kandelia candel and Rhizophora apiculata methanol extract of Kandelia candel exhibited highest anthelmintic activity by causing paralysis and death in the Pheretima Posthuma with good timing. This study provides scientific evidence that the leaves of Kandelia candel and Rhizophora apiculata have anthelmintic efficacy. Further study requires purification, characterization and structural elucidation of phytochemicals from these extracts that may help in the development of new drug formulations against various parasitic infections.