Author(s):

Sanjeev Ranjan, Bharat Bhushan, Jay Prakash Gupta and Manjit Panigrahi

Abstract: Mastitis is the most severe disease for dairy cattle based on traditional cost analysis. Genetic factor is one of the several factors affecting the occurrence of mastitis. Toll like receptor2 (TLR2), a key component of innate immune system, plays an important role in the initiation of the inflammatory responses to foreign pathogens. This present study was aimed to identify single nucleotide polymorphisms (SNPs) in the TLR2 gene and analyze the association of expression in different genotypes with mastitis. A total of two hundred nine crossbred cows (140 mastitis tolerant and 69 mastitis group) were selected to explore the genotypic and expression profiling of TLR2 gene and their association with mastitis susceptibility. Two fragments of TLR2 gene (179 bp and 313 bp) were amplified by Polymerase Chain Reaction (PCR). The different genotypic analysis by SSCP revealed that 179 bp fragment was monomorphic where in 313 bp was polymorphic. Association studies revealed that, AA genotype of 313 bp were less susceptible to mastitis and the mRNA expression of TLR2 was significantly (P<0.05) higher in AB and BB genotypic animals compare to AA. This suggested that genotype AA can be useful for selecting disease resistant traits.

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