Author(s):

Kuladip Prakash Shinde, Ramesh Chandra, Chand Ram Grover, Girish Panchbhai, Sunita Verma, Shailesh Kumar Gupta and Amit Kumar Singh

Abstract: The present experiment was carried out from November 2017 to April 2018 i.e. during the winter and summer season at ICAR-National Dairy Research Institute, Karnal (Haryana) to investigate the effect of supplementation of Lactobacillus plantarum CRD 2 and Lactobacillus rhamnosus CRD 9 probiotic cultures on physiological responses in Murrah buffalo calves. The new born Murrah buffalo calves were divided into four groups viz., T₁, T₂, T₃, and T₄. The calves of control group (T₁) were fed according to ICAR, 2013 feeding standard. Calves of T₂ were fed according to control group (T₁) except Lactobacillus plantarum CRD 2 supplementation 10⁸ cfu/ml (As fermented milk @ 100 ml/calf/day). The calves T₃ were fed according to control group (T₁) except Lactobacillus rhamnosus CRD 9 supplementation 10⁸ cfu/ml (As a fermented milk @ 100 ml/calf/day), also the calves of T₄ fed according to control group (T₁) except combination of Lactobacillus plantarum CRD 2 and Lactobacillus rhamnosus CRD 9 10⁸ cfu/ml [1:1 Ratio] (As a fermented milk @ 100 ml/calf/day) supplementation. During the winter season the overall mean± SE values of maximum and minimum temperature were 21.49±0.88 and 08.18±0.85 respectively while during the summer season the overall mean± SE values of maximum and minimum temperature were 30.22±1.74 and 13.45±1.38 respectively. The mean temperature-humidity index (THI) at morning and afternoon sessions during the winter season were 58.49±1.43 and 60.54±3.45 respectively while 75.07±1.72 and 59.20±1.29 respectively during the summer seasons. There were no significant (P<0.05) differences observed inrectal temperature, respiration rate and pulse ratebetween different treatment groups at the morning and afternoon session during the winter as well as summer season also during the pre-ruminant as well as post-ruminant phase while the rectal temperature, respiration and pulse rate in buffalo calves at afternoon session was slightly higher in all groups during the winter as well as summer season.There were also no significant (P<0.05) differences observed inrectal temperature, respiration rate and pulse ratebetween different treatment groups of female and male buffalo calves in the morning as well as afternoon session. The rectal temperature and respiration rate of treatment groups in the morning and at afternoon session in the both sexes was slightly lower than control group. The all observation were falls in the normal physiological range so these findings indicated that probiotic supplementation had no adverse effect on physiology of calves resulted in normalphysiological responses in buffalo calves, so probiotic supplementation advantageous in the removal of stress in the young buffalo calves during the stress period which are more prone to cold as well as heat stress.

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