Author(s):

Yogesh Pandey, Md Asad Khan, Syed Hassan Mehdi, Satish Chandra Sati, Preeti Bhatt and Chandra Kala Pant

Abstract: Breast cancer is reported as the most frequent tumor with limited treatments among the female worldwide. Galangin, bioflavonoids, has been shown anti-cancer properties in various cancer cells including breast cancer. Tumor necrosis factor alpha (TNF-α) is a proinflammatory cytokine that has been linked to breast cancer development. It strives multiple functions such as cell proliferation, differentiation and apoptosis. In this study, we investigated whether galangin could enhance TNF-mediated apoptosis in breast cancer cells. We further focused to investigate the molecular mechanism of inhibition of proliferation and apoptosis induced by the synergistic effect of galangin and TNF against human breast cancer cells. Cytotoxicity induced by galangin and TNF alone and in combination was measured by MTT assay. Apoptosis was detected by DNA fragmentation assay and measured by real time PCR and western blotting. The study clearly showed the dose dependent cytotoxic effect of galangin in combination with TNF in all the breast cancer cells. The dying cells showed characteristics of apoptosis such as, DNA fragmentation in combined treatment with galangin and TNF in human breast cancer cells, compared to single treatments. Furthermore, we also found that treatment with galangin enhances TNF-induced apoptosis by increasing expression of apoptosis-related proteins including caspase-3, 8, 9 and Bax whereas by decreasing the expression of Bcl-2. The data in the present study clearly demonstrates that galangin sensitizes human breast cancer cells to TNF mediated apoptosis and could have a potential therapeutic significance in treating cancer.

Pages: 2024-2030  |  891 Views  234 Downloads

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