Pharmacognosy

Journal of Pharmacognosy and Phytochemistry

  • Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Login

NAAS Rating: 5.21

updates
NAAS Rating: 5.21 new

Journal of Pharmacognosy and Phytochemistry

Vol. 6, Special Issue 1 (2017)

Efficacy of Rhizobium rhizogenesstrains on hairy root transformation in Stevia rebaudiana L. Bertoni

Author(s): Shweta Singh, Priyanka Mishra, ZA Haider and Madhuparna Banerjee
Abstract: Two strains of R. rhizogenes, ATCC15834 and MTCC 532 were tested for their virulence on different explants of Stevia rebaudiana. In case of R. rhizogenes strain ATCC 15834, out of different co-culture period, best results were observed after 48h treatment with maximum mean percentage of root induction on shoot tips (91.66%) as well as maximum mean number of hairy roots (20.6). In case of strain MTCC 532, 3h co-culture period was found to be best with maximum mean percentage of root induction (94.33%) and maximum mean number of hairy root (24.33). The overall study revealed that R. rhizogenes strain MTCC 532 was more effective than ATCC 15834 in terms of hairy root induction in S. rebaudiana. However, both the strains of R. rhizogenes (15834 and 532) were able to produce hairy roots in S. rebaudiana, where strain MTCC 532 was found better in terms of transformation frequency (94.33 %) as well as number of roots per explants (24.33).
Pages: 55-60  |  1108 Views  13 Downloads
How to cite this article:
Shweta Singh, Priyanka Mishra, ZA Haider and Madhuparna Banerjee. Efficacy of Rhizobium rhizogenesstrains on hairy root transformation in Stevia rebaudiana L. Bertoni. 2017; 6(6S): 55-60.
Call for book chapter
Journals List Click Here Research Journals Research Journals
Journal of Pharmacognosy and Phytochemistry