Journal of Pharmacognosy and Phytochemistry
Vol. 1, Issue 3 (2012)
Anti-TB Activity of Sterculia setigera Del., Leaves (Sterculiaceae)
Ibrahim T. Babalola, Esther A. Adelakun*, Yuehong Wang, Francis O. Shode
The use of herbs (medicinal plants) for the treatment of diseases is as old as the history of mankind. Sterculia setigera is an example of such plant endowed in nature to serve the needs of man. Folkloric claims on antibacterial activity of S. setigera were investigated in-vitro on a micro-scale using the Alamar Blue Assay. Three of four successive solvent extractions of the plant leaves extracts inhibited the growth of a virulent strain of Mycobacterium tuberculosis, H37Rv (ATCC27294) in the concentrations tested (1-128 Âµg/ml). The minimum inhibitory concentration (MIC) determined for the hexane, dichloromethane and ethyl acetate extracts were 84 Âµg/ml, 62 Âµg/ml and 128 Âµg/ml respectively. The combined active extracts were subjected to an extensive bioactivity-guided fractionation using a combination of normal phase (NP VLC) and reverse phase (RP MPLC) chromatography. Purification of active fractions led to pure sub-fractions with good activity (MICs 15.13 Âµg/ml-31.43 Âµg/ml). The cytotoxicity effect of the most active fractions was determined against African green monkey kidney cells (Vero cells) and human hepatocellular carcinoma (HepG2) cell lines. The fractions were non-toxic to Vero and HepG2 cells. Two pure compounds have been isolated from these active sub-fractions and structural elucidation is being carried out. The results show that S. setigera leaves are active against M. tuberculosis, and the anti-TB activity of ethnomedicinally used S. setigera leave has been confirmed by in vitro tests for the first time. This current report further suggests the presence of potential anti-mycobacterial agent(s) or anti-TB drug leads in S. setigera leaves.
Pages: 17-23 | 2186 Views 220 Downloads
Ibrahim T. Babalola, Esther A. Adelakun*, Yuehong Wang, Francis O. Shode. Anti-TB Activity of Sterculia setigera Del., Leaves (Sterculiaceae). J Pharmacogn Phytochem 2012;1(3):17-23.