Author(s):

Mala Agarwal*, Raka Kamal

Abstract: The explants from in vitro grown seedling were cultured on modified MS medium. Shoot, root and callus differentiation was obtained on MS medium supplemented with BAP, IBA and 2, 4-D respectively. Shoot as well as root differentiation was obtained on medium containing BAP+IBA/NAA. Multiple shoots with roots were obtained on MS medium without hormones(MSO).On Qualitative and quantitative estimation of flavonoids & steroids , presence of three flavonoids and three steroids was detected and the maximum amount was in 6-wk old callus cultures(2.90mg/g dw and 27.34mg/gdw respectively). while in different in vitro morphogenetic stages, the maximum amount of flavonoid and steroid content was in multiple shoots(2.96mg/gdw and 10.39mg/gdw).On supplementing the callus with different concentrations of cholesterol and salicylic acid significant recovery of diosgenin 6-wk old callus supplemented with 0.05mM concentration.

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