Author(s):

Mr. Hemanth Kumar 1*, M. Spandana

Abstract: Coleus forskolii variety of Nepal has got very less concentration of forskolin <0.13 % ± 0.05. A new and highly specific method has developed by Phytolabs in order to extract a well purified forskolin from the C. forskolii variety of Nepal. A rapid and validated method was developed for the evaluation of forskolin in Coleus forskohlii. Forskolin was quantitated by reverse-phase High performance liquid chromatography (HPLC) with a photodiode array detector at 210 nm. The temperature was held constant at 30 OC, and the retention time of forskolin was approximately 6.9min with purity of 82 % when compared with 70 % standard provided by GSN pharma. The samples were extracted with Acetonitrile by sonication. TLC has confirmed the forskolin with Rf value 0.25±0.02 evaluated through validated method.

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