Journal of Pharmacognosy and Phytochemistry
Vol. 2, Issue 4 (2013)
Evaluation of Cytotoxic and Anthelmintic Activity of Plant Roots of Clerodendrum viscosum (Family: Verbenaceae)
Md. Muksadur Rahman1, Md. Shahid Sarwar2, Abhijit Das1, Md. Mizanur Rahman Moghal1*, Md. Hasanuzzaman1
In the present study, in-vitro cytotoxic and anthelmintic activity of root extract of C. viscosum was evaluated. The Brine shrimp lethality bioassay method was used to determine the cytotoxic activity and vincristine sulphate was used as positive control. The plant extracts were also evaluated for in-vitro anthelmintic activity against Bangladeshi earthworms Pheretima posthuma and the time taken for worm paralysis and death was determined. One way analysis of variance (ANOVA) was used to determine the level of significance. The methanolic crude extract of plant root exhibited mild to moderate cytotoxicity with LC50 value of 10.235Â±1.061 mg/ml (95% CI: 7.60-12.87) as compared with vincristine sulphate with LC50 value of 0.841Â±0.025 mg/ml (95% CI: 0.78-0.90) and the difference was statistically significant (p<0.05). In the screening of anthelmintic activity, methanolic crude extract as well as aqueous fraction (water) of plant root revealed statistically significant anthelmintic activity on adult Bangladeshi earth worm Pheretima posthuma in comparison to the reference drug (p<0.05). The paralyzed time of methanolic crude extract, aqueous fraction and standard Albendazole were 13.23Â±0.49, 78.24Â±0.17 and 17.67Â±0.54 minutes at a concentration of 50 mg/ml. The death times of previous three samples were 45.10Â±1.79, 177.37Â±0.62 and 48Â±0.47 minutes respectively at the same concentration. In conclusion, our study suggests that the crude extracts of C. viscosum roots possess some action of cytotoxic and anthelmintic activities.
Pages: 119-122 | 1863 Views 234 Downloads
Md. Muksadur Rahman1, Md. Shahid Sarwar2, Abhijit Das1, Md. Mizanur Rahman Moghal1*, Md. Hasanuzzaman1. Evaluation of Cytotoxic and Anthelmintic Activity of Plant Roots of Clerodendrum viscosum (Family: Verbenaceae). J Pharmacogn Phytochem 2013;2(4):119-122.